Cell Meter™ Fluorimetric Cell Cytotoxicity Assay Kit


The measurement of mitochondrial dehydrogenases (e.g. LDH) activity is a well-accepted assay to quantify cell numbers and monitor cell viability. This cell viability assay kit provides a fast, simple, accurate and homogeneous assay for the fluorometric detection of viable cells.

This assay is based on the observation that blue and non-fluorescent resazurin is reduced to a pink fluorescent dye (resorufin) by accepting the electron from mitochondrial respiratory chain in live cells. The amount of resorufin produced is directly proportional to the number of living cells. The detection sensitivity of cell proliferation and cytotoxicity assays using this kit is higher than other assays such as MTT.
The characteristics of its high sensitivity (<100 CHO cells), non-radioactive and no-wash method made the kit suitable for high throughput screening of cell proliferation or cytotoxicity against a variety of compounds and adaptable for a wide variety of instrument platforms. The kit provides all the essential components with an optimized assay protocol. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.

Key Features :

Non-Radioactive          : No special requirements for waste treatment.
Continuous                 : Easily adapted to automation with no mixing or separation required.
Convenient                  : Formulated to have minimal hands-on time.
Variety applications      : Cell proliferation and cytotoxicity.
Sensitive and accurate : As low as 100 cells can be accurately quantified.
Enhanced value           : Less expensive than the sum of individual components.

Kit Components :

Component A : Assay Solution                             20 mL

1000 assays (96-well) / 2000 assays (384-well)

Brief Summary

→ Prepare cells with test compounds (100 μL /96-well-plate or 50 μL 384-well-plate)
→ Add 1/5 volume Assay solution → Incubate at room temperature for 1-4 hrs
→ Read Fluorescence at Ex 540/Em 590

References :

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