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 HOME > Cytokine Assays > Rat |
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Rat GM-CSF Kit |
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코 드 |
: 2201 |
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단 위 |
: 1 kit |
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공 급 원 |
: Bioo |
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가 격 |
: 문의 |
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granulocyte macrophage colony stimulating factor (GM-CSF)
Rat mature granulocyte macrophage colony stimulating factor (GM-CSF) is a polypeptide of 127 amino acids with one potential N-linked glycosylation site and two intramolecular disulfide bonds.
Natural rat GM-CSF is a monomeric glycoprotein with an apparent molecular mass ranging from 27 - 44 kDa. Rat GM-CSF shares 69% and 62% amino acid sequence identity with mouse and human GM-CSF, respectively. While rat and mouse GM-CSF have reciprocal cross-species activity, they are not active on human cells. GM-CSF is produced by a number of type II alveolar cells, renal mesangial cells, and endothelial cells. It is a pleiotrophic cytokine that stimulates the proliferation, differentiation, migration and survival of hematopoietic progenitor cells of the neutrophil, eosinophil, macrophage, megakaryocyte and erythroid lineages. GM-CSF activates the effector functions of mature neutrophils, eosinophils and macrophages.
In concert with IL-4 and other cytokines, GM-CSF induces dendritic cell formation and stimulates their ability to present antigens to cells of the immune system. In addition, GM-CSF inhibits Langerhans cell IL-12 production, thus promoting a generalized Th2-type response. Rat GM-CSF shares 69% and 62% amino acid sequence identity with mouse and human GM-CSF, respectively. While rat and mouse GM-CSFs have reciprocal cross-species activity, they are not active on human cells. Normal mouse and rat sera GM-CSF is undetectable (less than 15.6 pg/mL) in the absence of underlying pathology. In mice and humans, the high-affinity receptor for GM-CSF is composed of a 50 kDa ligand binding alpha subunit (GMR) and a 120 kDa signal transducing beta subunit. Furthermore, syndecan-2 is also reported to be a co-receptor for GM-CSF. |
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Rat GM-CSF
The specifications of the
Rat GM-CSF ELISA Test Kit are as follows:
Format: 96-well strip plate
Assay range: 16-1000 pg/ml
Sensitivity: 8 pg/ml
Total Assay Time: 3 hour and 45 minutes
Sample Size: 100 µl/well
Sample types: Serum, plasma and Cell Culture Supernatant
Wavelength for plate reading: A450 nm
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Product Code |
Unit |
Price |
Availability |
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2201 |
16-100 pg/ml |
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