Rat IL-10, 2206







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	Rat IL-10


	Rat IL-10 Rat Interleukin-10 (IL-10), also referred to as human cytokine synthesis inhibitory factor (CSIF), is a ~18 kDa protein of 160 amino acids (aa) after cleavage of an 18 aa signal peptide. Within the mature protein, there are two potential N-linked glycosylation sites and five cysteine residues. Four of the five cysteine residues are involved in the formation of two intrachain disulfide linkages that are essential for activity. Based on human and murine studies, biologically active rat interleukin-10 is likely to be a nondisulfide-linked homodimer in solution. IL-10 has pleiotropic effects in immunoregulation and inflammation. This cytokine is produced primarily by monocytes and to a lesser extent by lymphocytes. IL-10 down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. IL-10 also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggest that IL-10 functions as an essential immunoregulator in the intestinal tract. Mature rat IL-10 shares 85% aa sequence identity with mouse and 74% aa sequence identity with human IL-10. Rat and human IL-10 are both active on mouse cells, however; mouse IL-10 is not active on human cells.

Rat IL-10

The specifications of the Rat IL-10 ELISA Test Kit are as follows:

Format: 96-well strip plate

Assay range: 60-3840 pg/ml

Sensitivity: 30 pg/ml

Total Assay Incubation Time: 3 hour and 45 minutes

Sample Size: 100 µl/well

Sample types: Serum, plasma and Cell Culture Supernatant

Wavelength for plate reading: A450 nm

Product Code	Unit	Price	Availability
2206	60-3840 pg/ml