Rat TNF-α ELISA Test Kit,2203







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	Rat TNF-α ELISA Test Kit


	Rat tumor necrosis factor-alpha Rat tumor necrosis factor-alpha (TNF-α) is primarily a homotrimeric 17 kDa protein, a potent mediator of inflammatory and metabolic functions. TNF-α is produced by lipopolysaccharide stimulated macrophages. It was originally detected as a highly cytotoxic cytokine for tumor cells. It causes tumor necrosis in vivo when injected into tumor bearing animal and shows cytolytic activity against tumor cells in vitro. Further, TNF-α has been implied as central mediator in shock induced by gram negative microorganisms. TNF-α functions as a central mediator in many inflammatory and immunological processes. It is induced by various products of micro-organisms and by various cytokines. Interestingly, it also induced on its turn the production of many cytokines. Signal transduction occurs via two types of TNF-receptors, the p55 and p75 TNF- receptors. The receptors differ strongly in their intracellular signaling pathways.

TNF-α

The specifications of the Rat TNF-α ELISA Test Kit are as follows:

Format: 96-well strip plate

Assay range: 20-2000 pg/ml

Sensitivity: 10 pg/ml

Total Assay Incubation Time: 3 hour and 45 minutes

Sample Size: 100 µl/well

Sample types: Serum, plasma and Cell Culture Supernatant

Wavelength for plate reading: A450 nm

Product Code	Unit	Price	Availability
2203	20-2000 pg/ml