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 HOME > Biolchemical Reagents > Biotins_Avidins |
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Biotin-X nitrilotriacetic acid, potassium salt |
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코 드 |
: 3009 |
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단 위 |
: 1 mg |
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공 급 원 |
: ABD |
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가 격 |
: 문의 |
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다운로드 |
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NTA biotin
In combination with fluorescent avidin conjugates, this NTA biotin derivative can be used for detecting polyhistidine-containing biomolecules such as fusion proteins. This NTA Biotin derivative is a bifunctional reagent that is used to detect histidine-tagged proteins immobilized.
The nitrilotriacetic acid is used to chelate a Ni(II) ion at four of its six coordination sites. The remaining two sites are available for binding to a histidine tag. The biotin functional group can then be detected using a streptavidin-horseradish peroxidase conjugate and chemiluminescence.
Using this biotinylated nitrilotriacetic acid, it is possible to detect less than 0.11 pmol of histidine-tagged Escherichia coli RNA polymerase sigma70 subunit. This reagent is also able to specifically detect His-tagged sigma70 from a whole cell lysate following SDS-PAGE and transfer to nitrocellulose.
Thereagent can be dissociated from the His-tagged protein at pH 4.8 and the blot can be reprobed with a monoclonal antibody for detection of different proteins on the same blot. |
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NTA biotin
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Product Code |
Unit |
Price |
Availability |
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3009 |
1 mg |
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