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 HOME > Functional Assays > Calcium Detection |
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Fluo-8, AM |
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코 드 |
: 21080 |
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단 위 |
: 1 mg |
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공 급 원 |
: AAT Bioquest |
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가 격 |
: 문의 |
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다운로드 |
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Fluor8am
Quest Fluo-8™, AM
Storage : -20℃
Ex (nm) : 494 Em (nm) : 517
MW : 1000 Solvent : DMSO
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. However, Fluo-3 AM and Fluo-4 AM are only moderately fluorescent in live cells upon esterase hydrolysis, and require harsh cell loading conditions to maximize their cellular calcium responses. Quest Fluo-8™ are developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelength of maximum excitation @ ~490 nm and maximum emission @ ~520 nm. Quest Fluo-8™ AM only requires room temperature while Fluo-3 AM
and Fluo-4 AM require 37 oC cell loading. In addition, Quest Fluo-8™ is 2 times brighter than Fluo-4 AM, and 4 times brighter than Fluo-3 AM. ABD Bioquest offers a set of our outstanding Quest Fluo-8™ reagents with different calcium binding affinities (Quest Fluo-8: Kd = 389 nM; Quest Fluo-8H: Kd = 232 nM; Quest Fluo-8L: Kd = 1.86 µM). We also offer versatile packing sizes to meet your special needs, e.g., 1 mg; 10x50 µg; 20x50 µg; HTS packages with no additional packaging charge.
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Fluo-8 AM
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Product Code |
Unit |
Price |
Availability |
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21080 |
1 mg |
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21081
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5x50ug
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21082
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10x50ug
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21083
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20x50ug
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