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HOME > Functional Assays > Luciferase Assay
Luciferase Reporter Gene Assay Kit
   
코    드 : 12518
단     위 : 1 plate
공 급 원 : ABD Bioquest
가     격 : 문의
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  luciferase activity
Amplite™ Luciferase Reporter Gene Assay Kit


Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase. The advantages of a luciferase assay are the high sensitivity, the absence of luciferase activity inside most of the cell types, the wide dynamic range, rapidity and low cost. The most versatile and common reporter gene is the luciferase of the North American firefly Photinus pyralis. The protein requires no posttranslational modification for enzyme activity. It is not even toxic in high concentration (in vivo) and can be used in pro- and eukaryotic cells. The firefly luciferase catalyzes the bioluminescent oxidation of luciferin in the presence of ATP, magnesium and oxygen. This Amplite™ Luciferase Reporter Gene Assay Kit uses a proprietary luminogenic formulation to quantify luciferase activity in live cells and cell extracts. Our formulation generates a luminescent product that gives strong luminescence upon interaction with luciferase.


Key Features

Sensitive : Can detect as low as 0.1 pg luciferase/well.
Continuous : Stable luminescence, suitable for manual or automated operations with no mixing or separations
                     required.
Convenient : Formulated to have minimal hands-on time.
Non-Radioactive : No special requirements for waste treatment.

Kit Components

Component A : Luciferase Sensor (Light-sensitive)     1 vial
Component B : Assay buffer                                      1 vial (10 ml))

Brief Summary

→ Prepare cells (samples) with test compounds (100 μL 96-well-plate or 25 μL 96-well-plate)
→ Add equal volume of luciferase assay solution
→ Incubate at room temperature for 10-20 min
→ Read luminescence

References

1. McElroy, W.D. (1947) The Energy Source for Bioluminescence in an isolated System. Proc. Natl. Acad. Sci.
    USA 33,342.
2. de Wet JR, Wood KV, Helinski DR, DeLuca M, (1985) Cloning of firefly luciferase cDNA and the expression of active
    luciferase in Escherichia coli, Proc. Natl. Acad. Sci USA 82,7870-7873.
3. Khan, H.A. (2003) Bioluminometric assay of ATP in mouse brain: Determinant factors for enhanced test sensitivity,
    J. Bioscience 28, 379-382.
4. Drew, B and C. Leeuwenburgh (2003) Method for measuring ATP production in isolated mitochondria: ATP
    production in brain and liver mitochondria fo Fischer-344 rats with age and caloric restriction, Am J. Physiol. Regul.
    Integr. Comp. Physiol., 285, R1260-R1268.
5. Hara, K. Y. and Mori, H. (2006) An efficient method for quantitative determination of cellular ATP synthetic activity,
    J Biomol Screen 11, 310-7.
6. Sun, Y. and Chai, T. C. (2006) Augmented extracellular ATP signaling in bladder urothelial cells from patients with
    interstitial cystitis Am J Physiol Cell Physiol 290, C27-34.
   
 
  luciferase Assay

Product Code

Unit

Price

Availability

12518

1 plate