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HOME > Cell Biology > Cytotoxicity
Cell Cytotoxicity Assay Kit *Colorimetric
   
코    드 : 22780
단     위 : 1000 assay
공 급 원 : AAT Bioquest
가     격 : 문의
다운로드

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  Colorimetric Cell Cytotoxicity Assay
Cell Meter™ Colorimetric Cell Cytotoxicity Assay Kit



This kit uses a proprietary water-soluble tetrazolium dye that produces a water-soluble formazan dye upon cellular reduction in the presence of an electron carrier (such as PMS). The tetrazolium salt is reduced by cellular dehydrogenases to an orange formazan product that is soluble in culture medium. The amount of formazan produced is directly proportional to the number of living cells. Our kit provides a more robust protocol than other commercial tetrazolium-based cytotoxicity assay kits. No pre-mixing of components is required for our kit since the tetrazolium dye solution is added directly to the cells. In addition, our kit has higher sensitivity than the assays using the other tetrazolium salts (such as MTT and XTT). It can be readily adapted for a wide variety of instrument platforms. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized assay protocol. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.


Key Features

Non-Radioactive          : No special requirements for waste treatment.
Continuous                 : Easily adapted to automation with no mixing or separation required.
Convenient                 : Formulated to have minimal hands-on time.
Variety applications      : Cell proliferation and cytotoxicity.
Sensitive and accurate : As low as 300 cells can be accurately quantified.

Kit Components

Component A : Assay Solution                             20 mL

Brief Summary

→ Prepare cells with test compounds (100 μL /96-well plate or 50 μL/384-well plate)
→ Add 1/5 volume Assay Solution (Component A)
→ Incubate at room temperature for 1-4 hrs
→ Read Absorbance ratio at 570 and 605 nm

References

1. Carlson MA. (2006) Technical note: assay of cell quantity in the fibroblast-populated collagen matrix with a tetrazolium reagent. Eur Cell Mater, 12, 44.
2. Hayashi S, Kobayashi T, Honda H. (2003) Simple and rapid cell growth assay using tetrazolium violet coloring method for screening of organic solvent tolerant bacteria. J Biosci Bioeng, 96, 360.
3. Berridge MV, Herst PM, Tan AS. (2005) Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev, 11, 127.
4. Frederiks WM, van Marle J, van Oven C, Comin-Anduix B, Cascante M. (2006) Improved localization of glucose-6-phosphate dehydrogenase activity in cells with 5-cyano-2,3- ditolyl-tetrazolium chloride as fluorescent redox dye reveals its cell cycle-dependent regulation. J Histochem Cytochem, 54, 47.
5. Barbu A, Welsh N. (2004) The use of tetrazolium salt-based methods for determination of islet cell viability in response to cytokines: a cautionary note. Diabetologia, 47, 2042.
6. Demehin AA, Abugo OO, Rifkind JM. (2001) The reduction of nitroblue tetrazolium by red blood cells: a measure of Red Cell membrane antioxidant capacity and hemoglobinmembrane binding sites. Free Radic Res, 34, 605.
7. Proctor LM, Souza AC. (2001) Method for enumeration of 5-cyano-2,3-ditoyl tetrazoliumchloride (CTC)-active cells and cell-specific CTC activity of benthic bacteria in riverine, estuarine and coastal sediments. JMicrobiol Methods, 43, 213.
8. Ye D, Pospisilik JA, Mathers DA. (2000) Nitroblue tetrazolium blocks BK channels in cerebrovascular smooth muscle cell membranes. Br J Pharmacol, 129, 1035.
   
 
  22780

Product Code

Unit

Price

Availability

22780

1000 assay