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HOME > Functional Assays > Neurological Enzymes
Acetylcholinesterase Assay Kit
   
코    드 : 11400
단     위 : 200 assay
공 급 원 : AAT Bioquest
가     격 : 문의
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  Acetylcholinesterase Assay Kit
Amplite™ Colorimetric Acetylcholinesterase Assay Kit


Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to terminate synaptic transmission. AChE has a very high catalytic activity ?each molecule of AChE degrades about 5000 molecules of acetylcholine per second. Acetylcholinesterase is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms, which possess similar catalytic properties, but differ in their oligomeric assembly and mode of attachment to the cell surface. This Amplite?Colorimetric Acetylcholinesterase Assay Kit provides a convenient method for the detecting AChE activity. The kit uses DTNB to quantify the thiolcholine produced from the hydrolysis of acetylthiolcholine by AChE. The absoprtion intensity of DTNB adduct is proportional to the formation of thiolcholine, thus the AChE activity.


Key Features

Application  : Can be used to quantify acetylcholinesterase in solutions and in cell extracts.
Sensitive    : Detect as low as 0.1 mU of acetylcholinesterase in solution.
Continuous : Easily adapted to automation without a separation step.
Convenient : Formulated to have minimal hands-on time.
Non-Radioactive : No special requirements for waste treatment.

Kit Components

Component A : DTNB                                      1 vial
Component B : Assay Buffer                           1 bottle (25 mL)
Component C : Acetylthiocholine                     1 vial
Component D : Acetylcholinesterase Standard  1 vial (5 units)

Brief Summary

→ Prepare AChE reaction mixture (50 μL)
→ Add AChE standards or AChE test samples (50 μL)
→ Incubate at room temperature for 10-30 minutes
→ Read absorbance at 410 ±5 nm

References

1. Kovarik, Z et al. (2003). Acetylcholinesterase active centre and gorge conformations analysed by combinatorial mutations and enantiomeric phosphonates. Biochem. J. (2003) 373, 33–40.
2. Ordentlich, A. et al. (1996). The Architecture of Human Acetylcholinesterase Active Center Probed by Interactions withSelected Organophosphate Inhibitors. J. Biol. Chem. 271 (20):11953–11962.
3. Magnottl, RA. et al. (1987). Measurement of Acetylcholinesterase in Erythrocytes in the Field. Clin. Chem. 33/10, 1731-1 735.
   
 
  11400

Product Code

Unit

Price

Availability

11400

200 assay