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HOME > Functional Assays > Neurological Enzymes
Acetylcholine Assay Kit
   
코    드 : 11403
단     위 : 200 assay
공 급 원 : AAT Bioquest
가     격 : 문의
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  Acetylcholine Assay Kit
Amplite™ Fluorimetric Acetylcholine Assay Kit


Choline and its metabolites are needed for three main physiological purposes: structural integrity and signaling roles for cell membranes, cholinergic neurotransmission (acetylcholine synthesis), and as a major source for methyl groups via its metabolite, trimethylglycine (betaine) that participates in the S-adenosylmethionine synthesis pathways. It plays an important role in the central nervous system as a precursor for acetylcholine and membrane phosphatidylcholine. This Amplite?Fluorimetric Choline Assay Kit provides one of the most sensitive methods for the quantifying choline. The kit uses Amplite Red?to quantify choline through choline oxidase-mediated enzyme coupling reactions.
The fluorescence intensity of Amplite Red™ is proportional to choline.


Key Features

Application  : Can be used to quantify acetylcholine in solutions and in cell extracts.
Sensitive    : Detect as low as 0.01 nmoles of acetylcholine in solution.
Continuous : Easily adapted to automation without a separation step.
Convenient : Formulated to have minimal hands-on time.
Non-Radioactive : No special requirements for waste treatment.

Kit Components

Component A :  Amplite Red™                         1vial
Component B :  Acetylcholine Probe                 2 bottles (lyophilized powder)
Component C :  Acetylcholine Standard            1vial
Component D :  Assay Buffer                           1 bottle (25 mL)
Component E :  DMSO                                     1vial (100μL)

Brief Summary

→ Prepare ACh Assay mixture (50 μL)
→ Add ACh standards or ACh test samples (50 μL)
→ Incubate at room temperature for 10-30 min
→ Read fluorescence intensity at Ex/Em = 540/590 nm

References

1. Kovarik, Z et al. (2003). Acetylcholinesterase active centre and gorge conformations analysed by combinatorial mutations and enantiomeric phosphonates. Biochem. J. (2003) 373, 33–40.
2. Ordentlich, A. et al. (1996). The Architecture of Human Acetylcholinesterase Active Center Probed by Interactions withSelected Organophosphate Inhibitors. J. Biol. Chem. 271 (20):11953–11962.
3. Magnottl, RA. et al. (1987). Measurement of Acetylcholinesterase in Erythrocytes in the Field. Clin. Chem. 33/10, 1731-1 735.
   
 
  11403

Product Code

Unit

Price

Availability

11403

200 assay