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HOME > Functional Assays > Neurological Enzymes
Acetylcholinesterase Assay Kit (Red)
   
코    드 : 11402
단     위 : 200 assay
공 급 원 : AAT Bioquest
가     격 : 문의
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  Acetylcholinesterase Assay Kit
Amplite™ Fluorimetric Acetylcholinesterase Assay Kit *Red Fluorescence*


Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to terminate synaptic transmission. AChE has a very high catalytic activity ?each molecule of AChE degrades about 5000 molecules of acetylcholine per second. Acetylcholinesterase is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms, which possess similar catalytic properties, but differ in their oligomeric assembly and mode of attachment to the cell surface. This Amplite?Fluorimetric Acetylcholinesterase Assay Kit provides one of the most sensitive methods for the detecting AChE activity. The kit uses Amplite Red?to quantify the choline produced from the hydrolysis of acetylcholine by AChE through choline oxidase-mediated enzyme coupling reactions. The fluorescence intensity of Amplite Red?is proportional to the formation of choline, thus the AChE activity.


Key Features

Application  : Can be used to quantify acetylcholinesterase in solutions and in cell extracts.
Sensitive    : Detect as low as 0.01mU of acetylcholinesterase in solution.
Continuous : Easily adapted to automation without a separation step.
Convenient : Formulated to have minimal hands-on time.
Non-Radioactive : No special requirements for waste treatment.

Kit Components

Component A: Amplite Red™                                1vial
Component B: Acetylcholinesterase Probe            2 bottles (lyophilized powder)
Component C: Acetylcholinesterase Standard       1vial (5 units)
Component D: Assay Buffer                                 1 bottle (25 mL)
Component E: DMSO                                          1vial (100 μL)

Brief Summary

→ Prepare AChE Assay mixture (50 μL)
→ Add AChE standards or AChE test samples (50 μL)
→ Incubate at room temperature for 10-30 min
→ Read fluorescence intensity at Ex/Em = 540/590 nm

References

1. Kovarik, Z et al. (2003). Acetylcholinesterase active centre and gorge conformations analysed by combinatorial mutations and enantiomeric phosphonates. Biochem. J. (2003) 373, 33–40.
2. Ordentlich, A. et al. (1996). The Architecture of Human Acetylcholinesterase Active Center Probed by Interactions withSelected Organophosphate Inhibitors. J. Biol. Chem. 271 (20):11953–11962.
3. Magnottl, RA. et al. (1987). Measurement of Acetylcholinesterase in Erythrocytes in the Field. Clin. Chem. 33/10, 1731-1 735.
   
 
  11402

Product Code

Unit

Price

Availability

11402

200 assay