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HOME > Cell Biology > Cell signaling
Aldehyde Quantitation Kit
   
ÄÚ    µå : 10051
´Ü     À§ : 200 assay
°ø ±Þ ¿ø : AAT Bioquest
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  Aldehyde Assay Kit
Amplite¢â Colorimetric Aldehyde Quantitation Kit


Very reactive aldehydes, namely 4-hydroxyalkenals, were first shown to be formed in autoxidizing chemical systems. It was subsequently shown that 4-hydroxyalkenals, particularly 4-hydroxynonenal, were formed in substantial amounts under biological conditions, i.e. during the peroxidation of lipids of liver microsomes incubated in the NADPH-Fe system. Many other aldehydes were also identified in peroxidizing liver microsomes or hepatocytes, e.g., alkanals , alk-2-enals, and 4-hydroxyalkenals.

Amplite¢â Colorimetric Aldehyde Quantitation kit uses a proprietary dye that generates a chromogenic product upon reacting with an aldehyde. The kit provides a sensitive, one-step colorimetric method to detect as little as 1 nanomole of aldehyde in a 100 ¥ìL assay volume (10 ¥ìM). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and readily adapted to automation without a separation step. Its signal can be easily read with an absorbance microplate reader at 405 or 550 nm.


Key Features

Application  : Can be used for quantifying aldehydes in a variety of applications such as carbohydrate, lipid chemistry,
                    as well as enzyme reactions.
Sensitive     :  Detect as low as 1 nanomole of aldehyde.
Continuous : Easily adapted to automation without a separation step.
Convenient : Formulated to have minimal hands-on time.
Non-Radioactive : No special requirements for waste treatment.

Kit Components

Component A : AldeView¢â Yellow                          2 bottles
Component B : Assay Solution                               1 bottle (10 mL)
Component C : Aldehyde Standard                         1 vial
Component D : Dilution Buffer                                 1 bottle (20 mL)

Brief Summary

¡æ Prepare Enzyme reaction (50 ¥ìL)
¡æ Add 2X AldeView¢â Yellow reaction mixture (50 ¥ìL)
¡æ Incubate at room temperature for 30-60 min
¡æ Read Absorbence at 405 or 550 nm

References

1. Trevor M. Kitson. (1985) High concentrations of aldehydes slow the reaction of cytoplasmic aldehyde
    dehydrogenase with thiol-group modifiers Biochem. J. 228, 765.
2. Crabb DW, Matsumoto M, Chang D, You M (2004). Overview of the role of alcohol dehydrogenase and aldehyde
    dehydrogenase and their variants in the genesis of alcohol-related pathology. The Proceedings of the Nutrition
    Society 63 (1): 49.
3. Steinmetz CG, Xie P, Weiner H, Hurley TD (1997). Structure of mitochondrial aldehyde dehydrogenase: the genetic
    component of ethanol aversion. Structure 5 (5): 701.
4. O'Donnell JM, Kudej RK, LaNoue KF, Vatner SF, Lewandowski ED. (2004) Limited transfer of cytosolic NADH into
    mitochondria at high cardiac workload. Am J Physiol Heart Circ Physiol, 286, H2237.
5. Zurek G, and karst U (2000). 2,4-Dinitro-3,5,6-trideuterophenylhydrazones for the quantitation of aldehydes and
    ketones in air samples by liquid chromatography-mass spectrometry. J of chromatography A, 869, 251.
6. Ou Z, Ogamo A, Guo L, Konda Y, Harigaya Y, and Nakagawa Y. (1995). Identification and quantitation of choline
    glycerophospholipids that contain aldehyde residues by fluometric high-performance liquid chromatography.
    Analytical biochemistry 227, 289.
   
 
  10051

Product Code

Unit

Fluorescene

Availability

10051

200 assay

Colorimetric

 

10052

200 assay

Fluorimetric

 

10053

200 assay

Colorimetric